The course aims to teach the laboratory techniques applied to investigate various aspects of immune responses in experimental and clinical transplantation research. The Nantes course will address several techniques used in the isolation, characterization and analysis of dendritic cells (DCs) both in mice and humans. DCs play a central role in the mechanisms of allograft rejection and tolerance. The INSERM U643 laboratory has a large experience in the study of human and rodent dendritic cells in transplantation models and in their basic biology.
Target group
PhD students, young scientists and physicians directly involved in laboratory research involving or planning to use DCs are encouraged to enroll. Previous basic knowledge on flow cytometry and cell culture techniques are necessary.
Course Programme
- conferences on basic aspects of the biology of DC subpopulations
- multicolor flow cytometry to analyze plasmacytoid DCs (pDCs) and conventional DCs (cDCs) subsets in human blood.
- purification of human blood pDCs and cDCs.
- generation of human monocyte-derived DCs.
- Immunohistology of human tissues to identify DCs.
- multicolor flow cytometry to analyze pDC and cDCs subsets in mouse spleen.
- purification of mouse pDCs and cDCs from spleen.
- generation of murine bone marrow-derived DCs.
Cost
The course is sponsored by ESOT (housing, lunches, registration) thus free of charge.
Basic Science Travel Grants
6 travel grants of up to 500 € are availble – to apply visit the section GRANTS/ASWARDS on www.esot.org